3-Cyano-7-hydroxycoumarin NEW
| Price | $81 |
| Package | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-09-23 |
Product Details
| Product Name: 3-Cyano-7-hydroxycoumarin | CAS No.: 19088-73-4 |
| Purity: 98.03% | Supply Ability: 10g |
| Release date: 2025/09/23 |
Product Introduction
Bioactivity
| Name | 3-Cyano-7-hydroxycoumarin |
| Description | 3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) is a macrophage inhibitor of macrophage inhibitory factor (MIF) interconjugate isomerase with a Ki of 2.9 μM. 3-Cyano-7-hydroxycoumarin belongs to the group of coumarin derivatives, which can be used as molecular probes and fluorescent dyes. |
| In vitro | Instructions I. Solution preparation 1. Preparation of mother solution and working solution: It can be dissolved in DMSO and further diluted to aqueous buffer solution as working solution in the experiment. II. Operation steps 1. Sample preparation Target molecule: This probe is mainly used to detect MIF activity, or as a general fluorescent dye. It can be used to study the role of MIF in inflammation, cancer and immune response. Concentration: For fluorescence experiments, a solution with a concentration of 1–10 μM is usually used, and the specific concentration is adjusted according to the sensitivity and requirements of the experiment. 2. Experimental conditions 1) Buffer selection: In order to study MIF inhibition, it is recommended to select a suitable buffer (such as phosphate buffer with a pH range of 7.0–7.5) to simulate physiological conditions. 2) Add 3-Cyano-7-hydroxycoumarin: Add an appropriate amount of 3-Cyano-7-hydroxycoumarin solution (usually 0.1–1 mM) to the sample. 3) Reaction temperature and time: Typically, the reaction temperature is room temperature to 37°C. The reaction time depends on the activity of the enzyme in the sample and is usually between 30 minutes and 1 hour. 4) Hydrogen peroxide (H₂O₂) is usually added as an oxidant to initiate the reaction. 5) Color change: After the reaction is completed, the product is usually brown or dark in color and can be quantitatively detected using colorimetric methods (such as UV-Vis spectrophotometer). 3. Fluorescence imaging 3-Cyano-7-hydroxycoumarin can be used as a fluorescent probe to track molecular interactions or the activity of biomolecules such as MIF in live cell imaging. Detection: Fluorescence intensity is quantified by fluorescence microplate reader, confocal microscopy or flow cytometry. 4. Inhibition assay For MIF tautomerase inhibition assay, 3-Cyano-7-hydroxycoumarin can be used to determine its inhibitory potency with a Ki value of 2.9 μM. Methods: The inhibition of MIF by 3-Cyano-7-hydroxycoumarin was evaluated by measuring the fluorescence quenching effect. Storage conditions 1) 3-Cyano-7-hydroxycoumarin should be stored in a dry, cool place at -20°C, away from light and moisture. 2) The solution should be freshly prepared and, if storage is required, should be stored at -20°C or below and away from light. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 15 mg/mL (80.15 mM), Sonication is recommended. |
| Keywords | MIF | 3-Cyano-7-hydroxycoumarin |
| Inhibitors Related | RDR03785 | MIF-IN-2 | 4-IPP | MIF098 | Iguratimod | (±)-CPSI-1306 |
| Related Compound Libraries | Bioactive Compound Library | Inhibitor Library | Bioactive Compounds Library Max |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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