Calcein NEW
| Price | $47 |
| Package | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-06-18 |
Product Details
| Product Name: Calcein | CAS No.: 1461-15-0 |
| Purity: ≥98% | Supply Ability: 10g |
| Release date: 2025/06/18 |
Product Introduction
Bioactivity
| Name | Calcein |
| Description | Calcein (Fluorexon) is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage, a complexometric indicator for titration of calcium ions with EDTA, and for the fluorometric determination of calcium. |
| Cell Research | Instructions I. Dissolution and preparation 1. Preparation of mother solution: Calcein is dissolved in anhydrous DMSO, ethanol or PBS. The dissolved concentration can be adjusted according to the requirements of the experiment, usually in the range of 1-10 mM. 2. Preparation of working solution: When used for cell staining, the dissolved Calcein is added to the cultured cells, usually at a concentration of 1-5 μM (the specific concentration needs to be optimized according to the experiment). II. Cell staining 1. After adding Calcein, the cells usually need to be incubated at 37°C for 30 minutes to 1 hour. 2. After labeling, it can be analyzed using a fluorescence microscope or flow cytometer. 3. Calcein dye enters the cell through the permeability of the cell membrane. In the presence of calcium ions, it binds to calcium ions and emits green fluorescence. III. Lipid vesicle leakage detection Calcein can be used to evaluate the integrity of the cell membrane in lipid vesicle leakage experiments. When the lipid vesicle membrane is damaged, Calcein leaks out of the vesicle, resulting in changes in the fluorescence signal. Therefore, it can be used as an indicator for detecting membrane leakage and membrane stability. IV. Calcium ion titration and determination: 1. Calcein can also be used as an indicator for EDTA calcium ion titration and fluorescence determination of calcium ions. Calcein binds to calcium ions to form a fluorescent complex, so by measuring the fluorescence intensity, the concentration of calcium ions in the solution can be inferred. 2. In the calcium ion determination experiment, the fluorescence intensity of Calcein is linearly related to the concentration of calcium ions, and can usually be detected under the condition of absorption/emission spectra of 490/515 nm. V. Removal of background signal When performing fluorescence imaging or flow cytometric analysis, appropriate washing steps can be used to remove unbound Calcein dye on the cell surface or in the culture medium to reduce background fluorescence. Notes: 1. Calcein solution should be stored at -20°C and protected from light. 2. Avoid repeated freezing and thawing to maintain its fluorescence stability. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 100 mg/mL (160.63 mM), Sonication is recommended. H2O : 5 mg/mL (8.03 mM), when pH is adjusted to 12 with NaOH. Sonication is recommended. |
| Keywords | Inhibitor | inhibit | Calcein |
| Related Compound Libraries | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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