DAF-2 DA NEW
| Price | $1237 |
| Package | 1mg |
| Supply Ability: | 10g |
| Update Time: | 2026-09-10 |
Product Details
| Product Name: DAF-2 DA | CAS No.: 205391-02-2 |
| Supply Ability: 10g | Release date: 2026/09/10 |
Product Introduction
Bioactivity
| Name | DAF-2 DA |
| Description | DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm. |
| In vitro | Steps for assessing total mitochondrial nitric oxide levels: 1. SH-SY5Y cells were seeded in duplicate at a density of 5 × 10^3 cells/well in black 96-well cell culture plates. 2. After gelsemium treatment, cells were incubated with DAF-2 DA (10 μM, 15 minutes) on an orbital shaker at room temperature in the dark. 3. After washing twice with HBSS (Sigma) using a Cytation 3 cell imaging multi-function microplate reader, the formation of a green fluorescent product was detected at 485 nm (excitation)/538 nm (emission). The fluorescence intensity is the total cellular nitric oxide level. [2] |
| Storage | Keep away from direct sunlight, Store at -20°C Shipping with blue ice/Shipping at ambient temperature. |
| Keywords | 5,6-Diaminofluorescein diacetat |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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