Z-IETD-AFC NEW
| Price | $60 | $197 | $347 |
| Package | 1mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-17 |
Product Details
| Product Name: Z-IETD-AFC | CAS No.: 219138-02-0 |
| Purity: 98.67% | Supply Ability: 10g |
| Release date: 2025/07/17 |
Product Introduction
Bioactivity
| Name | Z-IETD-AFC |
| Description | Z-IETD-AFC is a fluorescent substrate for caspase-8 and granzyme B. It can be used to quantify cysteinyl aspartate-8 or granzyme B activity. |
| Cell Research | Instructions a. Solution preparation: 1. Prepare Z-IETD-AFC powder, dissolve it in DMSO, prepare a 10 mM stock solution, store below - 15°C, and protect from light. Prepare DTT (1 M), EDTA (100 mM), and Tris buffer (20 mM, pH=7.4). 2. Mix 50 μL Z-IETD-AFC stock solution, 100 μL DTT, 400 μL EDTA, and 10 mL Tris buffer to prepare a 2x Caspase substrate detection solution, in which the final concentration of Z-IETD-AFC is 50 μM. b. Operation steps: 1. Sample processing: Mix samples or cell lysates containing Caspase-8 activity with an equal volume of 2x Caspase substrate detection solution and incubate at room temperature for at least 1 hour. 2. Detection and analysis: The fluorescence intensity was monitored using a fluorescence microplate reader with an excitation wavelength of 376 nm and an emission wavelength of 482 nm. The activity of Caspase-8 was quantified based on the changes in fluorescence intensity. |
| Storage | store at low temperature,keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 4 mg/mL (4.87 mM), Sonication is recommended. DMSO : 4 mg/mL (4.87 mM), Sonication is recommended. |
| Keywords | Z-IETD-AFC | ZIETDAFC | Z IETD AFC |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $1.10/1g |
VIP5Y
|
Shaanxi Dideu Medichem Co. Ltd
|
2021-05-25 | |
| $1980.00/50mg |
VIP2Y
|
TargetMol Chemicals Inc.
|
2025-08-21 | |
| $47.00/1mg |
VIP4Y
|
TargetMol Chemicals Inc.
|
2025-08-29 | |
| $1.10/1g |
VIP5Y
|
Shaanxi Dideu Medichem Co. Ltd
|
2021-05-25 | |
| $1.00/1g |
VIP6Y
|
Career Henan Chemical Co
|
2020-01-08 |
United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
INQUIRY