Mad2L2 (Mad2-like protein 2), also known as REV7. is a multifunctional protein involved in diverse cellular processes, including DNA damage repair, cell cycle regulation, and chromosome segregation. It acts as a critical component of the translesion synthesis (TLS) pathway, where it partners with DNA polymerase ζ (Polζ) to bypass DNA lesions during replication, thereby maintaining genomic stability. Additionally, Mad2L2 interacts with the shieldin complex in DNA double-strand break repair, influencing the choice between non-homologous end joining (NHEJ) and homologous recombination (HR).
Antibodies targeting Mad2L2 are essential tools for studying its roles in these pathways. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect Mad2L2 expression, localization, and interactions in various biological contexts. Dysregulation of Mad2L2 has been linked to cancer progression, chemoresistance, and genomic instability, making its antibodies valuable in cancer research and therapeutic development. These antibodies also help explore Mad2L2’s emerging functions in meiosis, neurodegeneration, and immune regulation. By enabling precise detection and functional analysis, Mad2L2 antibodies contribute to understanding cellular stress responses and developing strategies to target replication and repair vulnerabilities in diseases.