ChemicalBook >> CAS DataBase List >>5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
- CAS No.
- 6578-06-9
- Chemical Name:
- 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
- Synonyms
- BCIP;BCIP P-TOLUIDINE SALT;BClP;BCIP-T;abs9176;BCIP P-TOL;X-PHOS P-TOL;BCIP-TOLUIDINE;P-toluidinesalt;BCIP P-TOLUIDINE
- CBNumber:
- CB4854083
- Molecular Formula:
- C8H6BrClNO4P.C7H9N
- Molecular Weight:
- 433.63
- MDL Number:
- MFCD00040636
- MOL File:
- 6578-06-9.mol
- MSDS File:
- SDS
- TDS File:
- TDS
Last updated:2026-05-28 01:17:48
| Melting point | 194-195℃ |
|---|---|
| storage temp. | -20°C |
| solubility | DMF: 20 mg/mL |
| form | tablets |
| color | White to Orange to Green |
| InChI | InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 |
| InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| SMILES | C12C(Cl)=C(C=CC=1NC=C2OP(=O)(O)O)Br.C1(C)C=CC(N)=CC=1 |
| CAS DataBase Reference | 6578-06-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9) |
| UNSPSC Code | 12352204 |
| NACRES | NA.83 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |    GHS02,GHS07,GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H226-H312+H332-H319-H360D | |||||||||
| Precautionary statements | P201-P210-P261-P280-P308+P313-P370+P378 | |||||||||
| PPE | dust mask type N95 (US), Eyeshields, Gloves | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38-40 | |||||||||
| Safety Statements | 26 | |||||||||
| RIDADR | 2811 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-8-10 | |||||||||
| TSCA | TSCA listed | |||||||||
| HazardClass | 6.1 | |||||||||
| PackingGroup | III | |||||||||
| HS Code | 29339190 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| NFPA 704 |
|
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt price More Price(70)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | BCIPP-RO | BCIP 5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt | 6578-06-9 | 250mg | $227 | 2026-04-30 | Buy |
| Sigma-Aldrich | BCIPP-RO | BCIP 5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt | 6578-06-9 | 1g | $494 | 2026-04-30 | Buy |
| Sigma-Aldrich | B6777 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt BioReagent, for molecular biology, powder, ≥99% | 6578-06-9 | 100MG | $139 | 2026-04-30 | Buy |
| Sigma-Aldrich | B6777 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt BioReagent, for molecular biology, powder, ≥99% | 6578-06-9 | 500MG | $488 | 2026-04-30 | Buy |
| Sigma-Aldrich | B0274 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt tablet | 6578-06-9 | 10 tabs | $441 | 2026-04-30 | Buy |
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties, Uses, Production
Chemical Properties
White to light brown powder
Uses
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
General Description
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphataseactivity in solution.
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Preparation Products And Raw materials
Raw materials
Preparation Products
Global Suppliers ( 342)
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Jinan Shangbo Pharmaceutical Co., Ltd. | 18253163690 | yinxiuyang@shengquan.com | China | 63 | 58 |
| Suzhou Rosen Biotechnology Co.,Ltd. | 0512-87888687 13626191410 | rosenchemical@163.com | China | 279 | 58 |
| Shanghai JONLN Reagent Co., Ltd. | 400-0066400 13621662912 | 422131432@qq.com | China | 9970 | 55 |
| Jiangsu Hi-Pure Technology New Materials Co. Ltd. | 0512-62764072; 18051857768 | sales@hi-pure.cn | China | 44 | 58 |
| Guangzhou Yaoguang Technology Co., Ltd. | 13020159086 | yangjue@ygfuture.com | China | 101 | 58 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| INTATRADE GmbH | +49 3493/605464 | sales@intatrade.de | Germany | 3563 | 66 |
| future industrial shanghai co., ltd | 400-0066400 13621662912 | sales@jonln.com | China | 1989 | 65 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
View Latest Price from 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-16 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
|
1KG | 98% | 3500kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-09-02 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
|
$1.00-10.00 | 1kg | 99% | Henan Fengda Chemical Co., Ltd | ||
 |
2021-07-13 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
|
$10.00-15.00 | 1KG | 99%+ HPLC | Zhuozhou Wenxi import and Export Co., Ltd |
-
- 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
- $0.00
- 98%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
- $1.00-10.00
- 99%
- Henan Fengda Chemical Co., Ltd
-
- 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
- $10.00-15.00
- 99%+ HPLC
- Zhuozhou Wenxi import and Export Co., Ltd
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BROMO-4-CHLORO-3-INDOLYL PHOSPHATE MONO-(-TOLUIDINIUM) SALT
BCIP/INT SUBSTRATE
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BCIP-T
5-Bromo-4-chloro-3-indolylphosphate p-toluidine salt p.a.
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Phosphoric acid-Mono-(5-broMo-4-chloro-1H-indol-3-yl) ester · p-toluidine, BCIP · p-toluidine, X-Phosphate · p-toluidine
5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT (BCIP P-TOLUIDINE SALT)
5-BroMo-4-Bhloro-3-Indolylphosphate4-toluidinesalt
Toluidine blue(BCIP)
BroMo-4-chloro-3-indolyl-phosphate-p-toluidine-salt (BCIP) research grade
X-PHOSPHATE P-TOLUIDINE SALT
X-PHOS P-TOL
X-PHOS P-TOLUIDINE SALT
1h-indol-3-ol,5-bromo-4-chloro-,dihydrogenphosphate(ester),compd.with4-m
ethylbenzenamine(1:1)
PHOSPHORIC ACID-MONO-(5-BROMO-4-CHLORO-1H-INDOL-3-YL) ESTER P-TOLUIDINE
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BCIP 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE
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(BCIP-TOLUIDINE)5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE-P-TOLUIDINE SALT
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt >99%
X-Phos p-toluidine salt, BCIP p-toluidine salt
1H-Indol-3-ol, 5-bromo-4-chloro-, 3-(dihydrogen phosphate), compd. with 4-methylbenzenamine
BClP
ACETIC ACID GLACIAL ACS GRADE
5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt, micronized
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP,P-toluidien salt)
p-Toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt≥ 99% (HPLC)
BCIP,P-toluidien salt
