- DTNB
-
- $52.00
-
2026-09-10
- CAS:69-78-3
- Purity: 98.18%
- Supply Ability: 10g
|
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Basic information |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties |
| Melting point | 240-245 °C (dec.)(lit.) | | Boiling point | 671.9±55.0 °C(Predicted) | | bulk density | 290kg/m3 | | density | 1.67 | | refractive index | 1.6900 (estimate) | | storage temp. | Keep in dark place,Sealed in dry,Room Temperature | | solubility | ethanol: 8mg/mL | | form | Yellow solid | | pka | 1.57±0.25(Predicted) | | color | Pale Yellow to Light Yellow | | Water Solubility | Soluble in water and ethanol. | | Merck | 14,3551 | | BRN | 1896821 | | Stability: | Stable. Incompatible with strong bases, strong oxidizing agents. | | InChI | 1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20) | | InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N | | SMILES | OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O | | CAS DataBase Reference | 69-78-3(CAS DataBase Reference) | | EPA Substance Registry System | Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | RTECS | DG9650000 | | F | 10 | | TSCA | TSCA listed | | HS Code | 29309070 | | Storage Class | 11 - Combustible Solids |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis |
| Chemical Properties | Crystalline | | Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample. | | Uses | Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content. | | Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups. | | Definition | ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups. | | General Description | 5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3. | | Biological Activity | Cell permeable: no', 'Primary Target
Useful in photometric determination of thiols', 'Product does not compete with ATP.', 'Reversible: no |
| | 5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials |
|