bisBenziMide H 33342 trihydrochloride manufacturers
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| | bisBenziMide H 33342 trihydrochloride Basic information |
| Product Name: | bisBenziMide H 33342 trihydrochloride | | Synonyms: | Hoechst 33342 (trihydrochloride);Hoechst 33342 (hydrochloride);CS-1351;Hoechst-33342, HOE 33342;BISBENZIMIDE H 33342 TRIHYDROCHLORIDE;HOE 33342 TRIHYDROCHLORIDE;HOECHST-33342 TRIHYDROCHLORIDE;HOECHST33342 TRIHYDROCHLORIDE;HOE 33342 trihydrochloride;Hoechst 33342 trihydrochloride, >99%;98% (HPLC and TLC) | | CAS: | 875756-97-1 | | MF: | C27H29ClN6O | | MW: | 489.02 | | EINECS: | | | Product Categories: | | | Mol File: | 875756-97-1.mol |  |
| | bisBenziMide H 33342 trihydrochloride Chemical Properties |
| storage temp. | Inert atmosphere,Store in freezer, under -20°C | | solubility | Soluble in water, N,N-dimethylformamide,
methano | | form | A crystalline solid | | pka | 11.08 ± 0.69, most acidic, temperature:
25 °C; 7.66 ± 0.42, most basic(at 25℃) | | color | Yellow to green powder or solid | | PH | 1.7 (20°C) | | Appearance | Solid Powder | | Water Solubility | H2O: 20mg/mL
phosphate buffer: precipitates | | λmax | 350 nm (H2O/DNA); 357 nm
(MeOH/H+) | | Major Application | Photoresists | | Biological Applications | Detecting
β-amyloid (Aβ) plaques; detecting/measuring
microorganisms; identifying/counting cells;
studying multidrug resistance; apoptosis assay;
cell cycle assay; cell proliferation assay; measuring
membrane potential; treating disc degenerative
disease, erectile dysfunction | | InChIKey | OTMWRDPOAAYNCR-UHFFFAOYSA-N | | SMILES | CN1CCN(C2=CC=C3N=C(C4=CC=C5N=C(C6C=CC(OCC)=CC=6)NC5=C4)NC3=C2)CC1.Cl |
| WGK Germany | WGK 3 | | Storage Class | 11 - Combustible Solids |
| | bisBenziMide H 33342 trihydrochloride Usage And Synthesis |
| Description | Hoechst 33342 is a cell-permeable DNA dye that binds to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA. | | Uses | Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells. It has been used to detect BrdU incorporation into cells and in studying the initial stages apoptosis and cellcycle distribution., Chromosomes th at are dividing or replicating will not stain with this dye. ', 'Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm | | Biological Activity | Membrane-permeable, fluorescent DNA stains with low cytotoxicity th at intercalate in A-T regions of DNA. | | storage | -20°C | | Excitation / Emission Maximum | 350 nm/461 nm |
| | bisBenziMide H 33342 trihydrochloride Preparation Products And Raw materials |
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