Desthiobiotin-PEG4-Alkyne manufacturers
|
| | Desthiobiotin-PEG4-Alkyne Basic information |
| | Desthiobiotin-PEG4-Alkyne Chemical Properties |
| Boiling point | 649.0±50.0 °C(Predicted) | | density | 1.075±0.06 g/cm3(Predicted) | | pka | 14.93±0.60(Predicted) |
| | Desthiobiotin-PEG4-Alkyne Usage And Synthesis |
| Description | Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. | | Uses | Desthiobiotin-PEG4-alkyne is a biotinylated biochemical reagent, which can be utilized in conjunction with click chemistry for the selective labeling and enrichment of certain tRNAs[1]. | | References | [1] Krasheninina OA, et al., Amine-to-Azide Conversion on Native RNA via Metal-Free Diazotransfer Opens New Avenues for RNA Manipulations. Angew Chem Int Ed Engl. 2021 Mar 22;60(13):6970-6974. DOI:10.1002/anie.202015034 |
| | Desthiobiotin-PEG4-Alkyne Preparation Products And Raw materials |
|