5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt NEW

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt

Modify Date: 2024-01-03 17:04:16

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt structure	Common Name	5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
	CAS Number	129541-41-9	Molecular Weight	521.787
	Density	N/A	Boiling Point	732.2ºC at 760mmHg
	Molecular Formula	C20H26BrClN2O7	Melting Point	228-230°C
	MSDS	ChineseUSA	Flash Point	396.6ºC

Purity		Quantity		Budget		Inquiry

Use of 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].

• Properties

• Articles11

• Spectrum

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 Names

Name	5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
Synonym	More Synonyms

  Biological Activity

Description	X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
References	[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.

 Chemical & Physical Properties

Boiling Point	732.2ºC at 760mmHg
Melting Point	228-230°C
Molecular Formula	C20H26BrClN2O7
Molecular Weight	521.787
Flash Point	396.6ºC
Exact Mass	520.061157
PSA	135.07000
Storage condition	−20°C

 MSDS

MSDS(Chinese)

 Safety Information

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Risk Phrases	R36/37/38
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
HS Code	29339990

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