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| | Methoxyresorufin Basic information |
| Product Name: | Methoxyresorufin | | Synonyms: | 7-methoxyresorufin;O7-METHYLRESORUFINE;RESORUFIN 7-O-METHYL ETHER;7-METHOXY-3H-PHENOXAZIN-3-ONE;O(7)-METHYLRESORUFINE, FOR FLUOR-ESCENCE;3H-Phenoxazin-3-one, 7-methoxy-;METHOXYRESORUFIN [RESORUFIN METHYL ETHER];7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O7-Methylresorufin | | CAS: | 5725-89-3 | | MF: | C13H9NO3 | | MW: | 227.22 | | EINECS: | | | Product Categories: | | | Mol File: | 5725-89-3.mol |  |
| | Methoxyresorufin Chemical Properties |
| Melting point | ≥220 °C(lit.) | | Boiling point | 378.8±42.0 °C(Predicted) | | density | 1.33±0.1 g/cm3(Predicted) | | storage temp. | 2-8°C | | solubility | DMF: soluble | | pka | 1.42±0.20(Predicted) | | form | Powder | | color | Brown to reddish brown | | Appearance | Solid Powder | | BRN | 209529 | | InChI | 1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3 | | InChIKey | KNYYMGDYROYBRE-UHFFFAOYSA-N | | SMILES | COc1ccc2N=C3C=CC(=O)C=C3Oc2c1 |
| Safety Statements | 22-24/25 | | WGK Germany | 3 | | F | 3-8-10 | | Storage Class | 13 - Non Combustible Solids |
| | Methoxyresorufin Usage And Synthesis |
| Description | Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2. Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively. The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity. | | Uses | Used to differentiate isozymes of cytochrome P-450 | | Uses | Substrate for Cytochrome P-450. METHOXYRESORUFIN is used for various investigations including the differentiatiation of cytochrome P-450 isozymes. | | Uses | Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC). | | Biochem/physiol Actions | Fluorimetric substrate for cytochrome P450 linked enzymes. | | IC 50 | CYP1 | | References | [1] RICHARD T. MAYER . Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases[J]. Pesticide Biochemistry and Physiology, 1977, 7 4: Pages 349-354. DOI: 10.1016/0048-3575(77)90038-4
[2] PRATIBHA V. NERURKAR . Methoxyresorufin and benzyloxyresorufin: substrates preferentially metabolized by cytochromes P4501A2 AND 2B, respectively, in the rat and mouse[J]. Biochemical pharmacology, 1993, 46 5: Pages 933-943. DOI: 10.1016/0006-2952(93)90504-p
[3] M.V. CABALLERO . Fipronil induces CYP isoforms in rats[J]. Food and Chemical Toxicology, 2015, 83: Pages 215-221. DOI: 10.1016/j.fct.2015.06.019
[4] De Souza, F.I., Zumiotti, A.V., and Da Silva, C.F. Neuregulins 1-α and 1-β on the regeneration the peripheral nerves[J]. Acta Ortop Bras. | | Excitation / Emission Maximum | 571 nm/585 nm |
| | Methoxyresorufin Preparation Products And Raw materials |
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