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Energy Chemical |
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| | IMIDAZOLE HYDROCHLORIDE Basic information |
| | IMIDAZOLE HYDROCHLORIDE Chemical Properties |
| Melting point | 158-161 °C(lit.) | | storage temp. | Inert atmosphere,Room Temperature | | solubility | H2O: 50 mg/mL, clear, colorless | | form | solid | | color | Pale yellow | | PH | 9.0-10.0 | | BRN | 3678283 | | Stability: | Stable. Hygroscopic. Incompatible with strong oxidizing agents. | | Major Application | diagnostic assay manufacturing | | InChI | InChI=1S/C3H4N2.ClH/c1-2-5-3-4-1;/h1-3H,(H,4,5);1H | | InChIKey | JDIIGWSSTNUWGK-UHFFFAOYSA-N | | SMILES | C1NC=NC=1.Cl | | CAS DataBase Reference | 1467-16-9(CAS DataBase Reference) |
| Hazard Codes | Xi | | Risk Statements | 36/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | F | 3-10 | | HS Code | 2933299090 | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Eye Irrit. 2
Skin Irrit. 2 |
| | IMIDAZOLE HYDROCHLORIDE Usage And Synthesis |
| Chemical Properties | white powder | | Uses | Imidazole hydrochloride is a useful research chemical. | | Uses | Imidazole hydrochloride has been used:
- in double immunohistochemistry
- to determine the activity of octopine dehydrogenase (ODH) in Mytilus galloprovincialis
- to study the cytoskeletal features during in vitro fertilization after intracytoplasmic sperm injection (ICSI) among humans
- as a reagent in the synthesis of a probe for monitoring herpes simplex virus type 1 thymidine kinase (HSV1-tk) reporter gene expression in rodents
| | Synthesis | In a flask, 68.22 g of imidazole was suspended in 505 g of xylene. The mixture was heated to reflux and 5 g of the xylene/water azeotropic mixture was removed by distillation to achieve dehydration. Subsequently, the temperature of the reaction system was lowered to 66°C and 25.2 grams of phosgene was added dropwise at a rate of 50.4 grams/hour over 30 minutes. After about 15 minutes, the viscosity of the reaction mixture increased significantly, exhibiting a chewing gum-like consistency. During the phosgene dropwise addition, the by-product imidazole hydrochloride precipitated in the form of yellow globules. After maintaining this temperature and continuing stirring for 1 hour, the reaction temperature was raised to 130 °C, at which time the imidazole hydrochloride transformed into a brown molten state. The melt was discharged from the reaction system at 130°C and solidified to a dark green solid upon cooling. The supernatant xylene phase was cooled to 0 °C, the precipitated crystals were filtered and dried at 20 mbar and 50 °C. A white crystalline N,N'-carbonyl diimidazole with a small amount of black impurities (Hazen color number: 489) was finally obtained with a purity of 96.8%, corresponding to 70% of the theoretical yield. | | References | [1] Patent: US2005/272937, 2005, A1. Location in patent: Page/Page column 4
[2] Patent: US2005/272937, 2005, A1. Location in patent: Page/Page column 3-4; 5 |
| | IMIDAZOLE HYDROCHLORIDE Preparation Products And Raw materials |
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