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Nhs Ester

Nhs Ester structure

CAS No.: 150347-59-4

Chemical Name:: Nhs Ester

Synonyms: NHS ester;CFSE;CFDA SE;Carboxyfluorescein succinimidyl ester;CFSE (CFDA-SE);≥90% (HPCE);5(6)-FAM DA SE;-Cellstain-Cfse;5-FAM-NHS DIACETATE;CFSE, 10 mM in DMSO

CBNumber:: CB00127828

Molecular Formula:: C29H19NO11

Molecular Weight:: 557.46

MDL Number:: MFCD31812843

MOL File:: 150347-59-4.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-09-12 18:52:08

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Nhs Ester Properties

Melting point	152-154 °C(lit.)
storage temp.	-20°C
solubility	DMF: soluble
pka	6.45(at 25℃)
form	Solid
color	Off-white to yellow
Appearance	white solid
PH Range	Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
λmax	<300nm
Major Application	Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays
InChIKey	QSWYNOQSGMUDQZ-UHFFFAOYSA-N
SMILES	C12(OC(=O)C3=CC(C(=O)ON4C(=O)CCC4=O)=CC=C13)C1C=CC(OC(=O)C)=CC=1OC1C=C(OC(=O)C)C=CC2=1
UNSPSC Code	12352108
NACRES	NA.32

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H302-H315-H319-H335

Precautionary statements

P261-P280-P301+P312-P302+P352-P305+P351+P338

PPE

Eyeshields, Gloves, type N95 (US)

WGK Germany

8-10-21

HS Code

32129000

Storage Class

11 - Combustible Solids

NFPA 704

	0
2		0

Nhs Ester price More Price(40)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	21888	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)	150347-59-4	25mg	$426	2026-04-30	Buy
Sigma-Aldrich	21888	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)	150347-59-4	100mg	$1190	2026-04-30	Buy
Cayman Chemical	14456	CFDA-SE ≥95%(mixtureofregioisomers)	150347-59-4	10mg	$85	2026-04-30	Buy
Cayman Chemical	14456	CFDA-SE ≥95%(mixtureofregioisomers)	150347-59-4	25mg	$179	2026-04-30	Buy
Cayman Chemical	14456	CFDA-SE ≥95%(mixtureofregioisomers)	150347-59-4	50mg	$313	2026-04-30	Buy

Product number	Packaging	Price	Buy
21888	25mg	$426	Buy
21888	100mg	$1190	Buy
14456	10mg	$85	Buy
14456	25mg	$179	Buy
14456	50mg	$313	Buy

Nhs Ester Chemical Properties, Uses, Production

Description

(5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.

This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase.

Chemical Properties

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder

Uses

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.

Uses

Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein.

Uses

A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye.

General Description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Abs/Em Maxima

492/517 nm

Nhs Ester Preparation Products And Raw materials

Raw materials

Preparation Products

Nhs Ester Suppliers

Global Suppliers ( 240)

Supplier	Tel	Email	Country	ProdList	Advantage
Dalian Meilun Biotech Co., Ltd.	0411-62910999 13889544652	sales@meilune.com	China	4765	58
Shanghai Qianhao Biotechnology Co., LTD	13524877553	13524877553@163.com	China	122	58
Nodado Polysaccharide (Dalian) Pharmaceutical Technology Co., Ltd	13840935583 13840935583	nuodaduotang@163.com	China	52	58
J & K SCIENTIFIC LTD.	18210857532 18210857532	jkinfo@jkchemical.com	China	96815	76
Beijing Ouhe Technology Co., Ltd	13552068683 13522913783	2355560935@qq.com	China	11777	60
Nanjing Habo Medical Technology Co., Ltd.	13376080562 13376090521	sales@habotech.com	China	724	60
Chemsky (shanghai) International Co.,Ltd	021-50135380	shchemsky@sina.com	China	15350	60
Tianjin heowns Biochemical Technology Co., Ltd.	400 638 7771	sales@heowns.com	China	14412	57
Beijing HuaMeiHuLiBiological Chemical	010-56205725	waley188@sohu.com	China	12323	58
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-6206333 13167063860	anhua.mao@aladdin-e.com	China	29985	65

Supplier	Advantage
Dalian Meilun Biotech Co., Ltd.	58
Shanghai Qianhao Biotechnology Co., LTD	58
Nodado Polysaccharide (Dalian) Pharmaceutical Technology Co., Ltd	58
J & K SCIENTIFIC LTD.	76
Beijing Ouhe Technology Co., Ltd	60
Nanjing Habo Medical Technology Co., Ltd.	60
Chemsky (shanghai) International Co.,Ltd	60
Tianjin heowns Biochemical Technology Co., Ltd.	57
Beijing HuaMeiHuLiBiological Chemical	58
Shanghai Aladdin Bio-Chem Technology Co.,LTD	65

View Latest Price from Nhs Ester manufacturers

Update time	Product	Min. Order	Purity	Supply Ability	Manufacturer
2025-06-07	Tetrazine NHS ester 1616668-55-3	25mg	>99.00%	100mg	Xian confluore Biological Technology Co., Ltd.
2025-06-07	Cyanine3.5 NHS ester 2231670-86-1	25mg	>99.00%	25mg	Xian confluore Biological Technology Co., Ltd.
2025-06-07	Cyanine7 NHS ester 2408482-09-5	25mg	>95.00%	25mg	Xian confluore Biological Technology Co., Ltd.

Tetrazine NHS ester
1616668-55-3
$0.00
>99.00%
Xian confluore Biological Technology Co., Ltd.

Cyanine3.5 NHS ester
2231670-86-1
$0.00
>99.00%
Xian confluore Biological Technology Co., Ltd.

Cyanine7 NHS ester
2408482-09-5
$0.00
>95.00%
Xian confluore Biological Technology Co., Ltd.

5(6)-(N-Succinimidyloxycarbonyl)-3',6',O,O'-diacetylfluorescein Spectrum

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN(150347-59-4)¹HNMR

150347-59-4 (Nhs Ester) Related Search:

4,4'-Oxydiphenol gamma-Phenyl-gamma-butyrolactone 5-Carboxyfluorescein diacetate tert-Butyl 3-butenoate 3,5-Bis(chloromethyl)pyridine HCl

4-(2 5-Dimethoxyphenyl)butyric acid 97 5(6)-(N-Succinimidyloxycarbonyl)-3',6',O,O'-diacetylfluorescein 6-Carboxyfluorescein diacetate 5(6)-CFDA Fluorescein diacetate

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CARBOXYFLUORESCEIN DIACETATE, NHS ESTER 5(6)-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER 5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5-CARBOXYFLUORESCEIN-NHS DIACETATE 5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER 5-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXYSUCCINIMIDE ESTER 5-CARBOXY-FLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5-FAM-NHS DIACETATE ≥90% (HPCE) 5(6)-carboxyfluorescein diacetate N-succinimidyl este -Cellstain-Cfse 5-or6-(N-Succiimidyloxycarbonyl)-3',6'-O,O'-diacethylfluorescein 5-or6-(N-Succinimidyloxycarbonyl)-fluorescein3',6'-diacetate 3'',6''-O,O''-Diacetyl-5(6)-(N-succinimidyloxycarbonyl)-fluorescein 5(6)-FAM DA SE 1-[[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]- 3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic Acid 2,5-Dioxo-1-pyrrolidinyl Ester 5(6)-Carboxyfluorescein 3',6'-Diacetate N-SucciniMidyl Ester 5(6)-CFDA, SE [5(6)-Carboxyfluorescein diacetate, succiniMidyl ester] 5(6)-Carboxyfluorescein diacetate, succinimidyl ester(CFDA) Carboxyfluorescein diacetate, succinimidyl ester 5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], 2,5-pyrrolidinedione derivative 5-(6)-carboxy' Uorescein Diacetate Succinimidyl Ester CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* ReadiUse CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* 2,5-Dioxopyrrolidin-1-ylacetatecompoundwith3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyldiacetate(1:1) Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester Cell proliferation tracer fluorescence probe 5-or 6-(N-Succinimidyloxycarbonyl)-3', 6'-O,O'-diacetylfluorescein (CFSE) 5(6)-CFDA N-succinmidyl ester CFSE[5-(and 6)-Carboxyfluorescein diacetate 5(6)-CFDA N-succinimidyl ester (CFSE) 5(6)-CFDA N-succinmidyl este Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester Cell Proliferation Tracer Fluorescent Probe (CFDASE) 5(6)-CFDA N-succinimidyl ester (CFSE), Fluorogenic esterase substrate CFSE, 10 mM in DMSO 5(6)-CFDA, SE [5(6)-Carboxyfluorescein diacetate, SE] Fluorescent pH Indicator - 25 mg 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester 5(6)-CFDA N-succinmidyl ester) MCE CFSE (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester carboxydiacetic acidacylisoaminester 3H-Indolium, 2-[7-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3,5-heptatrien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-, inner salt (ACI) Carboxyfluorescein succinimidyl ester CFSE CFDA SE CFSE (CFDA-SE) NHS ester 5(6)-(N-Succinimidyloxycarbonyl)-3',6',O,O'-diacetylfluorescein 150347-59-4 C58H38N2O22 Csub2subsub9subHsub1subsub9subNOsub1subsub1sub Fluorescent Probes, Labels, Particles and Stains Detection Biochemicals and Reagents BioChemical marker